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How many replicates do you actually need?

Plan before you sequence, not after. Enter your target effect size and biological variability to get a starting-point sample size estimate for differential expression.

Minimum fold change you want to reliably detect

Desired statistical power

Significance threshold (α)

Biological coefficient of variation

Not sure? 0.4 is a reasonable default for most human tissue studies.

Expected average read count for genes of interest

50 is a typical baseline for moderately expressed genes at standard sequencing depth.

Samples per Group

Total Samples (2 groups)

Talk Through Your Study Design →

This is a standard approximation (normal approximation to a negative-binomial model), useful for early planning. For a final, submission-ready power analysis, we'd recommend cross-checking with a tool like RNASeqPower or PROPER — happy to walk through that together.

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